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目的:探讨小檗碱对甲状腺功能亢进(甲亢)性腹泻大鼠结肠及血浆中神经肽Y的影响,进而研究小檗碱治疗甲亢性腹泻的机制。方法:制备甲亢性腹泻大鼠模型。应用小檗碱对其干预,采用免疫组化方法测定甲亢性腹泻组、小檗碱治疗剂量组、健康组大鼠结肠神经肽Y的定位,采用酶联免疫方法检测各组大鼠血浆中神经肽Y含量的变化。结果:在结肠组织中,甲亢性腹泻组与健康对照组比较NPY表达明显增多,经小檗碱治疗后NPY表达较甲亢性腹泻组表达减少,接近健康对照组的表达。在血浆中,NPY的表达在甲亢性腹泻组与健康对照组中增多,经小檗碱治疗后NPY表达较甲亢性腹泻组表达减少,接近健康对照组的表达。结论:甲亢性腹泻大鼠结肠及血浆中NPY的表达明显增多,小檗碱治疗后NPY的表达又减少,接近健康对照组。NPY可能参与甲亢性腹泻的发生机制.
Objective: To investigate the effect of berberine on neuropeptide Y in colonic and plasma of hyperthyroidism diarrhea rats and to study the mechanism of berberine in treating hyperthyroidism diarrhea. Methods: Preparation of hyperthyroidism diarrhea rat model. The intervention of berberine was applied to detect the neuropeptide Y localization in rats with hyperthyroidism diarrhea, berberine treatment and healthy rats by immunohistochemical method. The plasma levels of nerve in each group were detected by enzyme-linked immunosorbent assay Changes in peptide Y content. Results: In colorectal tissue, the expression of NPY in hyperthyroidism diarrhea group was significantly higher than that in healthy control group. The expression of NPY in colonic diarrhea group was lower than that in hyperthyroid diarrhea group, and close to that in healthy control group. In plasma, the expression of NPY increased in hyperthyroidism diarrhea group and healthy control group, the expression of NPY decreased after treatment with berberine compared with hyperthyroidism diarrhea group, which was close to the expression of healthy control group. Conclusion: The expression of NPY in colonic and plasma of rats with hyperthyroidism diarrhea increased obviously. The expression of NPY decreased after berberine treatment, close to the healthy control group. NPY may be involved in the pathogenesis of hyperthyroidism diarrhea.