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观察基因枪肌肉注射IL 2 preS融合蛋白表达质粒 (pCWIIP)的基因免疫效率 ,为乙型肝炎基因免疫研究提供基础。方法 :设基因枪肌肉注射组和正常肌肉注射组 ,分别用pCWIIP质粒及对照质粒pCIIP 2和pCI免疫Balb c小鼠 ,免疫后第 4天 ,取肌肉组织通过免疫组化观察IL 2 preS在肌肉中的表达 ;另设基因枪、正常肌肉、皮下注射组分别免疫pCWIIP及对照质粒 ,在 2、4、6、8w眼后腔取血 ,通过间接ELISA方法检测血清中抗preS的IgG抗体水平。结果 :免疫组化结果显示 :基因枪肌肉注射组其IL 2 preS在肌肉中的表达明显高于正常肌肉组。间接ELISA检测结果表明 :抗preS的IgG抗体水平为基因枪 >正常肌肉组 >皮下注射组。结论 :在IL 2 preS融合蛋白表达质粒的基因免疫中 ,基因枪肌肉注射的方式优于肌肉和皮下注射的方式 ,具有快速、经济和安全的特性 ,将为今后的临床广泛应用提供依据。
To observe the gene immunization efficiency of intramuscular injection of IL2 preS fusion protein plasmid (pCWIIP) by gene gun, and to provide a basis for the study of hepatitis B gene immunization. METHODS: Balb c mice were immunized with pCWIIP plasmid and control plasmid pCIIP 2 and pCI respectively by gene gun intramuscular injection group and normal intramuscular injection group. On the 4th day after immunization, muscle tissue was harvested for immunohistochemistry to observe the effect of IL 2 preS on muscle The other two plasmids were immunized with pCWIIP and control plasmids respectively in the gunshot, normal muscle and subcutaneous injection groups, and blood was collected in the posterior cavities of 2,4,6,8w eyes. The serum levels of anti-preS IgG antibodies were detected by indirect ELISA. Results: The results of immunohistochemistry showed that the expression of IL 2 preS in muscle was significantly higher than that in normal muscular group. The results of indirect ELISA showed that the level of anti-preS IgG antibody was gene gun> normal muscle group> subcutaneous injection group. CONCLUSION: In the gene immunization of IL 2 preS fusion protein expression plasmid, the way of intramuscular injection by gene gun is superior to the way of intramuscular injection and subcutaneous injection. It has the characteristics of rapid, economic and safety and will provide the basis for clinical application in the future.