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目的探讨孕乳期补充维生素D(Vit D)对仔鼠免疫球蛋白及T细胞的影响。方法选取雌性SD大鼠24只,随机分为低、中、高剂量1,25-(OH)_2D_3组和对照组。受孕第17天起,用1 ml/kg~(-1)·d~(-1)灌胃,分别给予花生油稀释的1,25-(OH)_2D_3滴剂40、80、120 IU·ml~(-1)·kg~(-1)及等剂量花生油,至子代大鼠21 d断乳。每组随机选择6只(雌雄各半),测定22 d仔鼠血清1,25-(OH)_2D_3含量,血清免疫球蛋白A(IgA)、血清免疫球蛋白M(Ig M)、血清免疫球蛋白G(IgG)水平及CD_4~+、CD_8~+T淋巴细胞比例和CD_4~+/CD_8~+比值。结果 3组血清1,25-(OH)_2D_3含量均有所升高,中、高剂量组升高显著,差异有统计学意义(P<0.05),但组间比较差异无统计学意义(P>0.05);低、中剂量组仔鼠IgG含量明显升高(P<0.05),高剂量组变化差异无统计学意义(P>0.05),3组IgA及Ig M含量比较差异无统计学意义(P>0.05);3组CD_4~+T淋巴细胞比例升高,低、中剂量组比较差异有统计学意义(P<0.05),但组间比较差异无统计学意义(P>0.05);3组CD_8~+T淋巴细胞比例升高,但差异无统计学意义(P>0.05);3组CD_4~+/CD_8~+比值均有所升高(P<0.05),且中、高剂量组CD_4~+/CD_8~+比值更高(P<0.01),组间比较差异无统计学意义(P>0.05),但中剂量组均数更高。结论孕乳期补充Vit D有利于增强子代的细胞免疫功能,80 IU/d剂量(相当于成人800 ng/d)较为适宜,对体液免疫功能的影响需进一步探究。
Objective To investigate the effects of vitamin D during pregnancy on immunoglobulin and T cells in offspring. Methods Twenty-four female Sprague-Dawley rats were randomly divided into low, medium and high dose 1,25- (OH) _2D_3 groups and control group. From the 17th day of conception, the mice were given gavage with 1 ml / kg ~ (-1) d ~ (-1), and were given peanut oil diluted 1,25- (OH) _2D_3 drops 40,80,120 IU · ml ~ (-1) · kg ~ (-1) and the same dose of peanut oil, to the offspring of rats 21 d weaning. The serum 1,25- (OH) _2D_3 levels, serum immunoglobulin A (IgA), serum immunoglobulin M (Ig M), serum immunoglobulin (IgG), CD 4 +, CD 8 + T lymphocyte ratio and CD 4 + CD 8 + ratio. Results The levels of serum 1,25- (OH) _2D_3 in all three groups were significantly increased, while those in middle and high dose groups were significantly increased (P <0.05), but there was no significant difference between the two groups (P > 0.05). There was no significant difference in the high and low dose groups (P> 0.05), while there was no significant difference in the IgA and IgM levels between the three groups (P> 0.05). The proportion of CD_4 ~ + T lymphocytes increased in three groups, there was significant difference between low and middle dose groups (P <0.05), but there was no significant difference between two groups (P> 0.05). The ratio of CD_4 ~ + / CD_8 ~ + in three groups increased (P <0.05), and the proportion of CD_8 ~ + T lymphocytes increased in three groups (P <0.05) The ratio of CD_4 ~ + / CD_8 ~ + was higher (P <0.01). There was no significant difference between the two groups (P> 0.05), but the mean of middle dose group was higher. Conclusions Vitamin D supplementation during pregnancy can enhance the cellular immune function of the offsprings. The dose of 80 IU / d (equivalent to 800 ng / d in adults) is more suitable, and the effect on humoral immune function needs to be further explored.