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[目的]观察补气通络解毒方含药血清对人BbPG3胰腺癌细胞抑制作用。[方法]将补气通络解毒方含药血清和空白对照血清分别在体外培养人胰腺癌细胞系BbPG3,MTT法检测各时间段胰腺癌细胞的OD值和细胞增殖率,流式细胞仪检测血清G0/G1、S、G2/M期细胞数。[结果]培养至4h时,细胞存活率为93.5%,2组OD值差异无统计学意义;24h时,细胞存活率为71.2%,2组OD值差异有统计学意义(P<0.05);48h时,细胞存活率为46.9%,2组OD值差异有统计学意义(P<0.05);72h时,细胞增殖率为19.7%,2组OD值差异有统计学意义(P<0.01)。给药组S期百分比明显低于空白对照组,2组差异有统计学意义(P<0.05),空白对照组G0/G1期细胞百分比明显升高,2组比较差异有统计学意义(P<0.05)。G2/M期,2组比较差异无统计学意义,给药组PI值明显低于对照组,差异有统计学意义(P<0.05)。[结论]补气通络解毒方含药血清有明显抑制人胰腺癌细胞增殖的效应。
[Objective] To observe the inhibitory effect of Buqi Tongluo Jiedu Fang containing serum on human BbPG3 pancreatic cancer cells. [Method] The human pancreatic cancer cell line BbPG3 was cultured in vitro with the serum of Buqi Tongluo Jiedu Decoction and the blank control serum respectively. The OD value and cell proliferation rate of pancreatic cancer cells in each time period were detected by MTT assay and flow cytometry Serum G0 / G1, S, G2 / M phase cell number. [Results] The cell viability was 93.5% at 4h after culture. There was no significant difference in OD value between the two groups. The cell survival rate at 24 hours was 71.2%. There was a significant difference in OD value between the two groups (P <0.05). At 48h, the cell viability was 46.9%. There was a significant difference in OD value between the two groups (P <0.05). At 72h, the cell proliferation rate was 19.7%. There was significant difference in OD value between the two groups (P <0.01). The percentage of S phase in the treated group was significantly lower than that in the blank control group (P <0.05), and the percentage of cells in G0 / G1 phase in the blank control group was significantly increased (P < 0.05). G2 / M phase. There was no significant difference between the two groups (P <0.05). The PI value of the treated group was significantly lower than that of the control group (P <0.05). [Conclusion] Buqi Tongluo Jiedu Decoction serum can significantly inhibit the proliferation of human pancreatic cancer cells.