芴、萘、菲、芘的荧光光谱无论是激发或是发射光谱均有重叠,本文提出用三维偏最小二乘法,利用这些化合物激发和发射光谱信息,同时解析其混合物的组成。实验表明,这四种组份在pH为7的磷酸缓冲液中。浓度很低时符合双线性,且具加和性。激发和发射波长数分别取为31和40,对未知样品测定,相对误差小于7％。 Fluorescence spectra of fluorene, naphthalene, phenanthrene, and pyrene both have excitation or emission spectra overlapped. In this paper, three-dimensional partial least squares (PLS) method is used to stimulate and emit spectral information and to analyze the composition of the mixture. Experiments show that these four components in pH 7 phosphate buffer. Biconic at low concentrations, with additive. Excitation and emission wavelength were taken as 31 and 40, respectively, for unknown samples, the relative error is less than 7%.