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建立了高效液相色谱法同时测定12种食品添加剂(苯甲酸、山梨酸、安赛蜜、柠檬黄、日落黄、觅菜红、胭脂红、赤藓红、新红、酸性红、诱惑红、罗丹明B)的方法。样品经前处理后,以甲醇-0.02 mol/L乙酸铵溶液梯度洗脱,采用紫外检测器检测,检测波长为240 nm,流速为1.0 mL/min。12种食品添加剂在0.5~30 mg/L范围内线性关系良好,回归系数都在0.999以上,方法加标回收率为93.1%~106.9%,相对标准偏差为1.4%~8.2%,检出限为0.07~0.63 mg/kg。
A high performance liquid chromatography (HPLC) was developed for the simultaneous determination of 12 food additives (benzoic acid, sorbic acid, acesulfame, lemon yellow, sunset yellow, magenta, carmine, erythrosine, Rhodamine B) method. The sample was pretreated with methanol - 0.02 mol / L ammonium acetate gradient elution, detection by UV detector, detection wavelength of 240 nm, the flow rate of 1.0 mL / min. The linearity of 12 kinds of food additives in the range of 0.5-30 mg / L was good with the regression coefficients above 0.999. The recoveries were 93.1% -106.9% with relative standard deviations of 1.4% -8.2%. The detection limits were 0.07 ~ 0.63 mg / kg.