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本文提出一种新的光散射探针染料———铀试剂Ⅰ (ArsenazoⅠ ) ,在pH 3 2 9的条件下 ,ArsenazoⅠ本身只有极弱的光散射 ,它与蛋白质的结合物有强烈的共振光散射作用。在λ =4 0 0nm处 ,光散射有最大强度 ,光散射强度与蛋白质的浓度成正比。据此建立了一种测定蛋白质的新方法。该方法简便、快速、灵敏度高 ,对HSA的检测限达到 6 0ng·mL-1 ,线性范围达到 0～ 18μg·mL-1 ,用于人体血清样品的分析并同考马斯亮蓝法比较 ,取得了令人满意的结果。本文也研究了牛血清白蛋白 (BSA)、γ球蛋白、鸡蛋白蛋白、溶菌酶、胃蛋白酶与染料ArsonazoⅠ之间的作用 ,探讨了共振光散射的机理
Arsenazo I, a new light-scattering probe dye, is proposed. Under the condition of pH 3 2 9, Arsenazo I itself has only very weak light scattering, which strongly resonates with the protein conjugate Scattering effect. At λ = 400 nm, light scattering has the maximum intensity, and the light scattering intensity is proportional to the protein concentration. Based on this, a new method for protein determination was established. The method is simple, rapid and sensitive. The detection limit of HSA is 60ng · mL-1 and the linear range is 0 ~ 18μg · mL-1. The method was applied to the analysis of human serum samples and compared with Coomassie brilliant blue method. Satisfactory result. This paper also studied the interaction between bovine serum albumin (BSA), gamma globulin, egg albumin, lysozyme, pepsin and dye Arsonazo I, and discussed the mechanism of resonance light scattering