论文部分内容阅读
目的：观察亮氨酸脑啡肽（L－Enk）和脂多糖（LPS）对血管平滑肌细胞（VSMC）增殖的影响，并观察两者之间的相互作用及阿片受体阻滞剂纳络酮（Nal）的影响．方法：实验以离体培养的SD大鼠胸主动脉VSMC为模型．用四唑盐比色实验（MTT）法及3H－TdR掺入技术观察VSMC的增殖、DNA合成量的变化．结果：研究发现10-7kg／L～10－5kg／LLPS可显著促进VSMC的增殖及DNA的合成（P<0．05）．10-5mol／L～10-4mol／LL－Enk对VSMC的增殖及DNA的合成有抑制作用（P<0．01）．10－8mol／L～10－5mol／LNal对VSMC的增殖及DNA的合成无显著影响．L－Enk可抑制LPS促VSMC增殖的作用，此抑制作用可被Nal阻断．结论：LPS促进VSMC的增殖和DNA合成，L－Enk可抑制LPS的促VSMC增殖作用，该抑制作用是通过阿片受体介导的．
OBJECTIVE: To observe the effects of L-Enk and LPS on the proliferation of vascular smooth muscle cells (VSMCs) and to observe the interaction between the two and the opioid receptor blocker naloxone (Nal) influence. Methods: In vitro cultured rat aorta VSMC model of SD rats. The proliferation and the amount of DNA synthesis of VSMC were observed by MTT assay and 3H-TdR incorporation assay. Results: It was found that 10-7kg / L ~ 10-5kg / LLPS can significantly promote VSMC proliferation and DNA synthesis (P <0.05). 10-5mol / L ~ 10-4mol / LL-Enk inhibited VSMC proliferation and DNA synthesis (P <0.01). 10-8mol / L ~ 10-5mol / LNal VSMC proliferation and DNA synthesis had no significant effect. L-Enk can inhibit the proliferation of LPS-induced VSMC, this inhibition can be blocked by Nal. Conclusion: LPS can promote VSMC proliferation and DNA synthesis. L-Enk can inhibit LPS-induced VSMC proliferation, which is mediated by opioid receptors.