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本文报道了不同来源的枇杷茎尖离体培养成功的方法与条件。取自实生幼树的茎尖在MS+6-BA0.5+NAA0.1+GA_3 0.1～0.2(毫克/升)培养基上顺利展叶,而后转接到MS+6-BA0.25+IAA0.05(毫克/升)中抽枝,在无激素的1/2MS培养基中长根。而取自嫁接结果树的茎尖则需要更高的外源激素。部分品种可在上述培养基中展叶,但必须在MS+6-BA1.0+NAA0.25(毫克/升)的培养基中才能抽枝,它需要用100毫克/升的IBA处理12小时才能长根。谷胱甘肽在枇杷茎尖离体培养中作用不大,而初期的全黑暗培养可提高成功率。不同生长状态的芽培养成功率各不相同。 本文还探讨了影响枇杷茎尖苗增殖速度的条件,认为6-BA可以抑制顶端优势,促进腋芽萌发,根据新枝增殖率和正常率与6-BA浓度之间的数量关系,认为最适繁殖的6BA浓度为1.5毫克/升。
In this paper, we report the successful methods and conditions of in vitro culture of loquat stem from different sources. The tips from the seedlings of young saplings were successfully expanded on MS + 6-BA0.5 + NAA0.1 + GA_3 0.1-0.2 (mg / L) medium and then transferred to MS + 6-BA0.25 + IAA0 Twenty-five shoots were harvested at 0.05 mg / l, rooted in hormone-free 1/2 MS medium. The shoot tips derived from the grafted tree need higher exogenous hormones. Some cultivars can be unfolded in the above medium but must be primed in medium with MS + 6-BA1.0 + NAA0.25 (mg / L) which requires treatment with 100 mg / L IBA for 12 hours To grow roots. Glutathione has little effect on in vitro culture of loquat stem tips, while initial darkness culture can improve the success rate. The budding success rates of different growth states vary. This paper also discussed the conditions affecting the proliferation rate of loquat stem shoots, considered 6-BA can inhibit the top advantage and promote the axillary buds germination, according to the new branch proliferation rate and the normal rate and the number of 6-BA concentration relationship between that the most suitable breeding 6BA concentration of 1.5 mg / l.