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由单克隆抗体与细胞毒物质组成的免疫毒素已引起极大关注。毒素中以蓖麻毒素为最常用,它由二条肽链组成,起细胞毒作用的是A 链。从植物Geloniummultiflorum 种子分离得分子量为30道尔顿的天然植物蛋白质gelonin 也具有抑制蛋白质合成的作用,其结构及生物学性质与A 链相似,但体内毒性比蓖麻毒A链明显为低,可与抗体组成免疫毒素。体外实验表明,在培养初期加入gelonin 可抑制小鼠脾细胞对PHA、ConA 和LPS 的反应,抑制程度取决于gelonin 浓度以及所加进的促有丝分裂剂。当gelonin 为2μg/ml 时,
Immunotoxins composed of monoclonal antibodies and cytotoxic substances have attracted great attention. Among the toxins, ricin is most commonly used. It consists of two peptide chains and the A chain acts as a cytotoxic agent. Gelonin, a natural plant protein with a molecular weight of 30 Daltons isolated from seeds of the plant Gelonium multiflorum, also has an inhibitory effect on protein synthesis. Its structural and biological properties are similar to those of the A chain, but its toxicity in vivo is significantly lower than that of the ricin A chain. Constitutes immunotoxins with antibodies. In vitro experiments showed that the addition of gelonin at the early stage of culture inhibited the response of mouse splenocytes to PHA, ConA, and LPS. The degree of inhibition depends on the concentration of gelonin and the added mitogenic agent. When gelonin is 2μg/ml,