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应用逆转录ＰＣＲ方法从Ｈｅｌａ细胞中成功地克隆了编码自身核抗原Ｕ１ＲＮＰ７０ｋＤ多肽的全长基因，将此基因重组入原核表达载体系统ＰＧＥＸ－２Ｔ中，以限制性酶切及核苷酸测序鉴定扩增的ＤＮＡ片段，经计算机分析，基因全长１．３ｋｂ，编码４３７个氨基酸，并进行了基因同源性比较。结果表明，克隆得到的基因与Ｇｅｎｂａｎｋ报道的完全一致，蛋白质一级结构分析表明编码的７０ｋＤ多肽在羧基片段有３个可能的抗原性区域，该多肽的抗原表位可能主要存在于羧基半段上，为重组蛋白的表达及抗原表位的定位研究奠定基础。
The full-length gene encoding the self-nuclear antigen U1RNP70kD was successfully cloned from HeLa cells by reverse transcription-polymerase chain reaction (RT-PCR). The recombinant plasmid was cloned into prokaryotic expression vector PGEX-2T and identified by restriction enzyme digestion and nucleotide sequencing The amplified DNA fragment was analyzed by computer. The gene was 1.3kb in length and encoded 437 amino acids. The comparison of gene homology was carried out. The results showed that the cloned gene was identical with that reported in Genbank. The primary structural analysis of the protein revealed that the encoded 70 kD polypeptide had three possible antigenic regions in the carboxyl fragment, and the epitope of the polypeptide probably existed mainly in the carboxyl half , Which laid the foundation for the study of the expression of recombinant proteins and the localization of epitopes.