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目的:建立以高效液相色谱法测定黄芩苷胃内漂浮片含量的方法。方法:以Kromasil C18柱(150mm×4.6mm,5μm)为色谱柱,流动相为甲醇-0.2%磷酸水溶液(48∶52),检测波长为280nm,流速为1mL/min,柱温为30℃,进样量20μL。结果:黄芩苷在0.3092～1.8552μg范围内进样量与峰面积呈良好的线性关系,平均回收率为99.87%,RSD=0.5%。结论:本方法简便、准确、专属性强,可用于该制剂的含量测定。
Objective: To establish a method for the determination of baicalin floating tablets in stomach by high performance liquid chromatography. Methods: Kromasil C18 column (150 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of methanol-0.2% phosphoric acid solution (48:52), the detection wavelength was 280 nm, the flow rate was 1 mL / Injection volume 20μL. Results: There was a good linear relationship between baicalin and peak area in the range of 0.3092 ~ 1.8552μg with the average recovery of 99.87% and RSD 0.5%. Conclusion: The method is simple, accurate, specific and can be used for the determination of the content of the preparation.