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为建立体外抗ＨＢＶ 药物筛选的技术，应用乙肝病毒（ＨＢＶ）ＤＮＡ 转染细胞株２－２－１－５ 细胞培养上清中ＨＢｓＡｇ 和ＨＢｅＡｇ 滴度作为药物抗ＨＢＶ 效果的评价指标，对４ 种中药制剂的抗ＨＢＶ 作用进行了评价。同时，应用四甲基偶氮唑盐（ＭＴＴ） 比色分析法检测药物的细胞毒性。结果显示，从中草药中挖掘抗ＨＢＶ 的药物具有较大的潜力，而将ＨＢＶ ＤＮＡ 转染细胞株用于抗ＨＢＶ 药物体外筛选，是一个简单易行的方法。
To establish an in vitro anti-HBV drug screening technique, HBsAg and HBeAg titers in the culture supernatant of hepatitis B virus (HBV) DNA transfected cell line 2-2-1-5 cells were used as evaluation indicators for the anti-HBV efficacy of the drug, and for 4 Anti-HBV effects of TCM preparations were evaluated. At the same time, the cytotoxicity of the drug was detected by MTT colorimetric assay. The results show that there is a great potential for excavating anti-HBV drugs from Chinese herbs, and it is a simple and easy method to use HBV DNA transfected cell lines for anti-HBV drug screening in vitro.