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以超高产杂交水稻(Oryza sativa L.)“培矮64S/E32”和多年来大面积推广的杂交稻“汕优63”为材料,研究孕穗后剑叶中C4途径酶和对稳定碳同位素分异作用的变化。结果表明,籽粒灌浆期(移栽后68～75 d)的两个品种剑叶中NADP-MDH活性最高,随后下降;超高产杂交水稻“培矮64S/E32”的NADP-MDH的活性明显高于“汕优63”;PEPCase和NADP-ME活性在黄熟期之前的叶片中持续上升。不同生育期的叶片与籽粒的△13C值相近(19.49‰～19.82‰),在成熟期时较高。超高产水稻“培矮64S/E32”叶片的平均△13C值比“汕优63”高0.43‰。
In this study, the C4 pathway enzymes in flag leaves of bryo-baryon and the effects of stable carbon isotope fractionation on the yield and quality of hybrid rice “Pei’ai 64S / E32” (Oryza sativa L.) and the hybrid rice “Shanyou 63” popularized over the years Different effects of change. The results showed that the activities of NADP-MDH in the flag leaves of two cultivars at grain filling stage (68-75 d after transplanting) were the highest, and then decreased. The activity of NADP-MDH in “Peiai 64S / E32” In “Shanyou 63”, the activities of PEPCase and NADP-ME increased continuously in leaves before yellow ripening. The △ 13C values of leaves and grains at different growth stages were similar (19.49 ‰ ~ 19.82 ‰), but higher at maturity. The average △ 13C value of super high-yielding rice “Pei’ai 64S / E32” was 0.43 ‰ higher than that of “Shanyou 63”.