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[目的]观察和分析中药疏肝健脾方对腹泻型肠易激综合征(IBS-D)模型大鼠结肠神经生长因子(NGF)及其受体TrkA、P75表达的影响,以期阐述中药疏肝健脾方治疗IBS-D可能机制。[方法]将18只2日龄SD雄性幼鼠随机分为正常组、模型组、疏肝健脾组,除正常组外其余各组使用母婴分离加束缚应激的方法制备IBS-D大鼠模型。造模成功后正常组、模型组予生理盐水,疏肝健脾组予疏肝健脾方灌胃14d。取大鼠结肠黏膜,采用酶联免疫法检测NGF及其TrkA、P75表达。[结果]疏肝健脾方可以降低IBS-D模型大鼠结肠黏膜组织NGF含量(P>0.05)、5-HT含量(P<0.05),下调受体TrkA、P75的表达(P<0.05)。[结论]疏肝健脾方干预IBS-D模型大鼠,改善内脏敏感性及腹泻部分机制与调节NGF及其TrkA、P75表达有关。
[Objective] To observe and analyze the effect of Shugan Jianpi Recipe on the expression of nerve growth factor (NGF) and its receptors TrkA and P75 in rats with diarrhea-predominant irritable bowel syndrome (IBS-D) Mechanism of Liver-Spleen Recipe in Treating IBS-D. [Methods] Eighteen 2-day-old male SD rats were randomly divided into normal group, model group, Shuganjianpi group, except for the normal group, the other groups were prepared using maternal and infant separation plus restraint stress IBS-D large Mouse model. After the model was established, the normal group was given normal saline. The model group was treated with Shugan Jianpi Decoction for 14 days. The colon mucosa of rats were taken out and the expression of NGF and TrkA, P75 was detected by enzyme-linked immunosorbent assay. [Result] Shuganjianpi Decoction could reduce the expression of NGF (P> 0.05), 5-HT (P <0.05) and the expression of TrkA, P75 in colonic mucosa of IBS-D model rats . [Conclusion] The intervention of Shuganjianpi Prescription in IBS-D model rats can improve visceral sensitivity and some mechanism of diarrhea and regulate the expression of NGF and TrkA, P75.