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This study was conducted to assess the effects of difenoconazole (DFZ),a triazole fungicide,on the hepatic biotransformation system and its bioaccumulation in marine medaka (Oryzias melastigma).Fish were exposed to DFZ (1,10,100,1000 ng/L) for 180 days.The results showed that:(1) The mRNA levels of hepatic CYP1A1,CYP1B,CYP1C1,CYP27B and CYP3A40 were up-regulated,but those of CYP3A38 and CYP27A1 were down-regulated.(2) The activity of ethoxyresorufin-O-deethylase (EROD) and the content of reduced glutathione (GSH) in the liver were increased in the DFZ-treated groups,and glutathione S-transferase (GST) activity was increased in the 100 and 1000 ng/L groups.(3) DFZ was accumulated in the muscle and the biological concentration factors in the 10,100,and 1000 ng/L groups were respectively 149,81 and 25.These results suggested that long-term exposure to DFZ at low concentrations would result in a bioaccumulation of this compound and disturb the biotransformation system.