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目的探讨中国北方汉族人群HLA-DRB1、DQA1单倍型与乙型肝炎病毒(hepatitisBvirus,HBV)感染不同结局的关系。方法采用序列特异性引物聚合酶链反应(sequencespecificprimerspolymerasechainreaction,PCR-SSP)技术检测HLA-DRB1、DQA1等位基因,并比较207例慢性乙型肝炎患者,212名无症状HBV慢性携带者(HBV携带者),148例自限性HBV感染者的单倍型频率。结果自限性HBV感染组单倍型DRB1*04-DQA1*0301的频率为10.03%,显著高于慢性乙肝组的3.66%(P=0.0005);DRB1*15/*16-DQA1*0102的频率为6.80%,显著高于慢性乙肝组的1.94%(P=0.0012)和无症状HBV慢性携带者组的1.65%(P=0.004);DRB1*04-DQA1*0302单倍型在慢性乙型肝炎组的频率为3.10%,明显高于自限性HBV感染组的0.39%(P=0.0077)。结论HLA-DRB1、DQA1单倍型与个体感染HBV后的不同结局存在显著关联。
Objective To investigate the relationship between HLA-DRB1 and DQA1 haplotypes and different outcomes of hepatitis B virus (HBV) infection in northern Chinese Han population. Methods The HLA-DRB1 and DQA1 alleles were detected by sequence-specific primers polymerase chain reaction (PCR-SSP) and compared with 207 chronic hepatitis B patients and 212 asymptomatic HBV carriers ), Haplotype frequency in 148 cases of self-limited HBV infection. Results The frequencies of haplotype DRB1 * 04-DQA1 * 0301 in self-limited HBV infection group were 10.03%, significantly higher than those in chronic hepatitis B group (P = 0.0005). The frequency of DRB1 * 15 / * 16- Was 6.80%, significantly higher than 1.94% (P = 0.0012) of chronic hepatitis B group and 1.65% (P = 0.004) of chronic asymptomatic HBV carrier group; DRB1 * 04-DQA1 * 0302 haplotype in chronic hepatitis B The frequency of the group was 3.10%, significantly higher than that of the self-limited HBV infection group (0.39%, P = 0.0077). Conclusion The haplotypes of HLA-DRB1 and DQA1 are significantly associated with the different outcomes of individuals infected with HBV.