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目的:检测PDGF-BB是否可促进人类牙髓细胞成牙本质分化过程。方法:体外原代培养牙髓细胞,将PDGF-BB作用于人类牙髓间质细胞,检测牙髓间质细胞在矿化诱导培养基的分化。利用实时定量PCR检测分化相关指标牙本质涎磷蛋白(DSPP)及牙本质基质蛋白1(DMP1)的表达水平。利用免疫印迹技术检测PDGF-BB处理后的人牙髓间质细胞胞内AKT信号分子的活化情况。结果:PDGF-BB可明显促进人类牙髓间质细胞矿化结节的形成。同时,利用实时定量PCR检测发现巨噬细胞上清处理的牙髓间质细胞的DSPP及DMP1表达明显上调。且牙髓间质细胞内的AKT磷酸化水平明显升高。结论:PDGF-BB可促进牙髓间质细胞的成牙本质分化,可能在牙本质形成过程中发挥重要作用。
OBJECTIVE: To determine if PDGF-BB can promote the process of odontoblast differentiation in human dental pulp cells. Methods: Primary cultured human dental pulp cells were treated with PDGF-BB in human dental pulp cells to detect the differentiation of dental pulp cells in mineralization-induced medium. Real-time quantitative polymerase chain reaction (PCR) was used to detect the expression of dentin sialoprotein (DSPP) and dentin matrix protein 1 (DMP1). The activation of intracellular AKT signaling molecules in human dental pulp stromal cells treated with PDGF-BB was detected by Western blotting. Results: PDGF-BB significantly promoted the formation of mineralized nodules in human dental pulp stromal cells. Meanwhile, the expression of DSPP and DMP1 in dental pulp stromal cells treated with macrophage supernatant was significantly up-regulated by real-time PCR. AKT phosphorylation in dental pulp stromal cells was significantly increased. Conclusion: PDGF-BB can promote odontoblast differentiation of dental pulp cells and may play an important role in dentin formation.