聚丙烯酰胺凝胶电泳具有分辨力高、灵敏等特点,广泛应用于各种生物大分子的分析,成为强有力的实验手段。血红蛋白的珠蛋白肽链分析一般有:1.羧甲基纤维素柱层析法,2.以巯基乙醇—尿素为解离剂和以对氯汞苯甲酸为解离剂的电泳分析法。但有的操作繁杂。费时,有的分辨力低,不能分离HbF的~Gγ和~Aγ链。Rovera等结合巯基乙醇—尿素解离和含Triton X-100的酸性聚丙烯酰胺凝胶电泳,除可分析α链和β链外,~Gγ链和~Aγ链也可分开。Alter等利用此法进行了~Gγ和~Aγ比率的测定和肽链合成的研究,并应用于产前诊断β地中海贫血和α地中海贫血纯合子,有快速、简便、取样量比层析法少几百倍等优点。我们基本上采用Alter法,略加变动,在鉴别β~+和β°地中海贫血方面结果明确,可以纠 Polyacrylamide gel electrophoresis with high resolution, sensitivity and other characteristics, widely used in a variety of biological macromolecules analysis, a powerful experimental means. Hemoglobin globin peptide chain analysis are generally: 1. Carboxymethyl cellulose column chromatography, 2. To mercaptoethanol - urea as a dissociation agent and p-chloromercuribenzoic acid as a dissociation agent electrophoresis. However, some operations are complicated. Time-consuming, and some low resolution, can not separate ~ Gγ and ~ Aγ chain of HbF. Rovera et al., Combined with mercaptoethanol-urea dissociation and acidic polyacrylamide gel electrophoresis with Triton X-100, can separate the ~ Gamma and ~ A [gamma] chains in addition to the [alpha] and [beta] chains. Using this method, Alter et al. Have studied the determination of ~ Gγ and ~ Aγ ratios and the synthesis of peptide chains, and applied them to the prenatal diagnosis of β-thalassemia and α-thalassemia homozygote, which is quick and easy, and the sampling amount is less than that of chromatography Hundreds of times and other advantages. We basically use the Alter method, a slight change in the identification of β ~ + and β ° thalassemia results clear, you can correct