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目的:探讨流式细胞术在再生障碍性贫血(AA)和低增生型骨髓增生异常综合征(L-MDS)鉴别诊断中的临床价值。方法:采用多色流式细胞术检测5例L-MDS和7例AA患者骨髓粒细胞免疫表型,并对其血象和免疫表型进行比较。结果:L-MDS和AA患者外周血血红蛋白、造血岛个数、粒红淋系比例差异均存在显著统计学意义(P<0.01,P<0.05)。L-MDS和AA患者骨髓CD34+、CD117+细胞比例、粒系CD7表达、CD15缺失、CD45平均荧光强度(MFI)异常、CD13/CD16图形异常比例差异存在显著统计学意义(P<0.01,P<0.05)。结论:流式检测骨髓病态造血有助于L-MDS和AA的鉴别诊断。
Objective: To investigate the clinical value of flow cytometry in the differential diagnosis of aplastic anemia (AA) and low-proliferative myelodysplastic syndrome (L-MDS). Methods: The immunophenotypes of myeloid granulocytes in 5 cases of L-MDS and 7 cases of AA were detected by multi-color flow cytometry, and the blood phenotype and immunophenotype were compared. Results: There was a significant difference in the proportion of hemoglobin, hematopoietic island and erythroid cells between L-MDS and AA patients (P <0.01, P <0.05). The percentage of CD34 +, CD117 + cells, the expression of granulocyte CD7, CD15, CD45 average fluorescence intensity (MFI) and CD13 / CD16 abnormalities in L-MDS and AA patients were significantly different (P <0.01, P <0.05 ). Conclusion: Flow cytometry can help the differential diagnosis of L-MDS and AA.