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目的:检测MTA1和RECK基因在鼻咽癌(NPC)中的表达及其与NPC临床病理因素的关系。方法:采用原位杂交技术检测60例原发性NPC(颈淋巴结转移组和无颈淋巴结转移组各30例)、10例NPC颈部转移淋巴结(MLN)和20例慢性鼻咽炎组织(CNT)中MTA1和RECK mRNA的表达,并对检测结果与NPC临床病理因素进行统计学分析。结果:①CNT、NPC和MLN中MTA1的阳性表达率分别为30.0%(6/20)、71.7%(43/60)和80.0%(8/10),与CNT比较,NPC和MLN中MTA1表达上调,差异均有统计学意义(均P<0.05)。NPC淋巴结转移组与无淋巴结转移组MTA1阳性表达率分别为83.3%(25/30)和60.0%(18/30),差异有统计学意义(P<0.05)。②CNT、NPC和MLN中RECK的阳性表达率分别为85.0%(17/20)、26.7%(16/60)和30.0%(3/10)。与CNT比较,NPC和MLN中RECK表达下调,差异均有统计学意义(均P<0.05)。NPC淋巴结转移组与无淋巴结转移组RECK阳性表达率分别为13.3%(4/30)和40.0%(12/30),差异有统计学意义(P<0.05)。③NPC中MTA1和RECK异常表达与患者性别、年龄、T分期和临床分期无关(均P>0.05),与颈淋巴结转移、复发和治疗后5年生存期有关(均P<0.05),且二者表达呈负相关(r=-0.541,P<0.05)。结论:MTA1和RECK基因异常表达可能与NPC的进展有关,共同参与了NPC的侵袭转移过程。MTA1和RECK可作为评估NPC颈淋巴转移、复发及预后的生物学参考指标。
Objective: To detect the expression of MTA1 and RECK in nasopharyngeal carcinoma (NPC) and its relationship with the clinicopathological factors of NPC. Methods: Sixty cases of primary NPC (30 cases of cervical lymph node metastasis and no cervical lymph node metastasis), 10 cases of NPC neck lymph node (MLN) and 20 cases of chronic nasopharyngitis (CNT) were detected by in situ hybridization. In MTA1 and RECK mRNA expression, and test results and NPC clinical and pathological factors for statistical analysis. Results: ① The positive expression rate of MTA1 in CNT, NPC and MLN was 30.0% (6/20), 71.7% (43/60) and 80.0% (8/10), respectively. Compared with CNT, the expression of MTA1 in NPC and MLN was up-regulated , The differences were statistically significant (all P <0.05). The positive expression rates of MTA1 in NPC and non-lymph node metastasis groups were 83.3% (25/30) and 60.0% (18/30) respectively, with statistical significance (P <0.05). ② The positive rates of RECK in CNT, NPC and MLN were 85.0% (17/20), 26.7% (16/60) and 30.0% (3/10), respectively. Compared with CNT, the expression of RECK in NPC and MLN was down-regulated, with statistical significance (all P <0.05). The positive expression rates of RECK in NPC and non-lymph node metastasis groups were 13.3% (4/30) and 40.0% (12/30), respectively, with statistical significance (P <0.05). (3) The abnormal expression of MTA1 and RECK in NPC was not related to the gender, age, T stage and clinical stage (all P> 0.05), but also to the 5-year survival rate after cervical lymph node metastasis, recurrence and treatment (both P <0.05) The expression was negatively correlated (r = -0.541, P <0.05). Conclusion: Aberrant expression of MTA1 and RECK genes may be related to the progress of NPC and participate in the invasion and metastasis of NPC. MTA1 and RECK can be used as a biological reference to evaluate NPC neck lymph node metastasis, recurrence and prognosis.