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在妊娠第7、8、9天经口给孕鼠不同剂量的前体物亚硝酸钠(分别为0.125、0.25、0.50、1.00和2.00mmol/kgBW)和乙基脲(剂量是亚硝酸钠的二倍),同时给浓缩猕猴桃汁或4％淀粉液。不给猕猴桃汁的各组随前体物剂量增大,活胎率下降,吸收胎率逐渐增加,分别为5.21％,43.66％,71.70％,85.80％和100％。最高剂量组胚胎全部死亡,并有半数孕鼠中毒死亡。单给高剂量亚硝酸钠或乙基脲则不引起胚胎或孕鼠死亡。表明亚硝酸钠和乙基脲在大鼠体内合成N-亚硝基乙基脲,并通过胎盘引起胚胎死亡。前体物与胚胎毒性之间有明显的剂量反应关系。同时给孕鼠浓缩猕猴桃汁的各组在亚硝酸钠0.125-0.50mmol/kgBW者胚胎存活率无明显减少,仅最高剂量组活胎率明显减少,吸收胎率达58.6％,但无孕鼠中毒死亡。实验结果表明浓缩猕猴桃汁能明显阻断大鼠体内N-亚硝基乙基脲合成,预防所引起的胚胎毒性。
Pregnant rats were orally administered different doses of precursors sodium nitrite (0.125, 0.25, 0.50, 1.00 and 2.00 mmol / kg BW, respectively) and ethyl urea (doses of sodium nitrite Twice), while giving concentrated kiwi juice or 4% starch solution. In each group that did not give kiwi juice, the dosages of precursors increased, the live birth rate decreased, and the absorbed birth rate increased by 5.21%, 43.66%, 71.70%, 85.80% and 100% respectively. The highest dose group of embryos all died, and half of pregnant rats poisoning death. Single doses of sodium nitrite or ethyl urea did not cause embryo or pregnant mouse deaths. It is shown that sodium nitrite and ethylurea synthesize N-nitrosoethylurea in rats and cause embryonic death through the placenta. There is a clear dose-response relationship between precursor and embryotoxicity. In the meantime, the survival rate of embryos of 0.125-0.50mmol / kgBW sodium nitrite was not significantly reduced in all groups of pregnant rats with concentrated kiwifruit juice. The highest rate of live births was significantly reduced in the highest dose group, with a 58.6% death. The experimental results show that concentrated kiwifruit juice can obviously block the synthesis of N-nitrosoethylurea and prevent embryotoxicity in rats.