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用水迷宫和一次性被动回避反应两种行为学训练方法 ,结合脑室内注射一氧化氮合酶 (NOS)抑制剂 NAME(Nω-nitro-L -arginine)和原位杂交技术 ,观察了阻断 NOS前后大鼠海马和大脑皮层前额叶等区域由行为学训练诱发的生长抑素 (somatostatin,SOM) m RNA阳性细胞数量的改变。结果显示 :(1)同未经训练的对照组相比 ,两种行为学训练都引起海马和大脑皮层中 SOMm RNA阳性细胞的显著增加 ;(2 )在脑室中注射了 NAME的实验组动物 ,两种行为学训练都不能再诱发上述阳性细胞的增加 ,同时NAME也阻止了训练组出现的学习和记忆的形成。以上结果提示 ,一氧化氮参与了作为脑内学习和记忆神经化学基础之一的生长抑素表达增加的调控
Water maze and one-time passive avoidance reaction behavioral training methods, combined with intracerebroventricular injection of nitric oxide synthase (NOS) inhibitor NAME (Nω-nitro-L-arginine) and in situ hybridization was observed blocking NOS Changes in the number of somatostatin (SOM) m RNA positive cells induced by behavioral training in the hippocampus and prefrontal cortex of the rat cortex before and after. The results showed that: (1) Both behavioral training induced a significant increase of SOMm RNA positive cells in hippocampus and cerebral cortex compared with untreated control group; (2) experimental group injected NAME into ventricles, Neither behavioral training was able to induce the above-mentioned increase of positive cells, meanwhile, NAME also prevented the formation of learning and memory in training group. The above results suggest that nitric oxide is involved in the regulation of increased somatostatin expression as one of the foundations of neural learning and memory neurophysiology in the brain