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探讨新疆传统发酵乳酪乳清中生物活性肽(简称乳清活性肽)对炎症状态下3T3-1小鼠脂肪细胞PPARγmR-NA表达及NF-κB活性的影响,并探讨其可能的作用机制。体外培养小鼠胚胎成纤维细胞3T3-L1,经0.15 mmol/L 3-异丁基-1-甲基黄嘌呤、0.125μmol/L地塞米松和10 mg/L胰岛素诱导成为成熟的脂肪细胞后,给予脂多糖(LPS)刺激使脂肪细胞成炎症模型,再给予乳清活性肽(1,10,20,50 mg/L),以及阳性对照药高密度脂蛋白(10,50,100 mg/L),测定脂肪细胞中PPARγmRNA表达及NF-κB的活性。PPARγ在分化成熟的脂肪细胞有较高水平的表达,LPS诱导后表达下降,PPARγ的表达在HDL和乳清活性肽干预后明显升高,并且乳清活性肽20,50 mg/L组、HDL 50,100 mg/L组与LPS致炎组相比有统计学差异;NF-κB活性在LPS致炎后升高,与正常组相比有统计学意义(P<0.05),HDL和乳清活性肽干预组中NF-κB活性明显减低,并且乳清活性肽20,50 mg/L浓度组,HDL 50,100 mg/L组与LPS致炎组相比有统计学意义(P<0.05)。乳清活性肽可能是通过上调PPARγmRNA的表达以及降低NF-κB活性来发挥抗动脉粥样硬化的作用。
To investigate the effect of bioactive peptides (whey active peptide) from traditional Chinese fermented whey in Xinjiang on the expression of PPARγmR-NA and the activity of NF-κB in adipocytes of 3T3-1 mice under inflammatory conditions and to explore its possible mechanism. Mouse embryonic fibroblasts 3T3-L1 were cultured in vitro and induced to mature adipocytes after 0.15 mmol / L 3-isobutyl-1-methylxanthine, 0.125 μmol / L dexamethasone and 10 mg / L insulin (1, 10, 20 and 50 mg / L) and positive control high-density lipoprotein (10, 50 and 100 mg / L) The expression of PPARγmRNA and the activity of NF-κB in adipocytes were measured. The expression of PPARγ in differentiated mature adipocytes was higher than that in LPS and the expression of PPARγ was significantly increased after the intervention of HDL and whey active peptide. The levels of NF-κB in LPS-treated group were significantly higher than that in LPS-treated group (P <0.05), and the levels of HDL and whey active peptide The activity of NF-κB in the intervention group was significantly decreased, and there was statistical significance (P <0.05) in the concentrations of serum active peptide 20,50 mg / L, HDL 50,100 mg / L and LPS-induced inflammation group. Whey active peptide may play an anti-atherosclerotic role by up-regulating PPARγ mRNA expression and decreasing NF-κB activity.