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用富含成骨细胞的小鼠头盖骨细胞测试肿瘤坏死因子(TNF)对骨细胞的作用以及蛋白激酶C(PK C)抑制剂H-7对TNF的影响.方法:[~3H]TdR参入法观察细胞增殖.以对-硝基苯酚磷酸酯为底物用微量分光光度法测定细胞内碱性磷酸酶和耐酒石酸酸性磷酸酶活性.结果:TNF(1—100 kU·L~(-1))以剂量依赖方式抑制骨细胞增殖和碱性磷酸酶活性,但增强耐酒石酸酸性磷酸酶活性.H-7(5—20μmol·L~(-1))对TNF上述作用具有显著抑制作用且呈剂量依赖.结论:TNF对骨细胞的作用与PK C有关.
The effects of tumor necrosis factor (TNF) on osteocytes and the effect of protein kinase C (PKC) inhibitor H-7 on TNF were assayed by using osteoblast-rich mouse osteoblasts.Methods: [~ 3H] TdR incorporation The activity of alkaline phosphatase and tartrate-resistant acid phosphatase (ALT) in cells was determined by the micro-spectrophotometry with p-nitrophenol phosphate as substrate.RESULTS: TNF (1-100 kU · L -1) ) Inhibited osteoblast proliferation and alkaline phosphatase activity in a dose-dependent manner, but increased tartrate-resistant acid phosphatase activity.H-7 (5-20μmol·L -1) significantly inhibited the above effects of TNF Dose-dependent.Conclusion: The effect of TNF on osteocytes is related to PKC.