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为使细胞融合顺利,选择适当的促融剂显得十分重要。目前常用的促融剂有灭活了的仙台病毒,溶血卵磷脂和聚乙二醇(Po-lyethylene glycol)。灭活仙台病毒和溶血卵磷脂这两种促融剂制备困难,诱导活性不稳定。聚乙二醇(简称 PEG)是人工合成药剂,它具有高融合性,且每批融合活性始终如一,融合效率稳定,对细胞毒性亦较低,使用简单,成为细胞触合的良好促融剂,大有取代其他促融剂的趋势。本文是“细胞融合技术”的续篇,报导以 PEG 作为诱导剂的细胞融合技术。
For the cell to fuse smoothly, it is very important to choose the appropriate flux. At present, the commonly used fluxes are inactivated Sendai virus, lysolecithin and polyethylene glycol (Po-ly ethylene glycol). The inactivation of Sendai virus and lysolecithin, both of which are difficult to prepare, have unstable induction activities. Polyethylene glycol (abbreviated as PEG) is a synthetic agent, it has high fusion, and each batch of fusion activity is consistent, the fusion efficiency is stable, and the cytotoxicity is also low. It is simple to use and becomes a good foaming agent for cell contact. There is a strong tendency to replace other accelerating agents. This article is a continuation of the “cell fusion technology” and reports cell fusion technology using PEG as an inducer.