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目的:筛选出五味沙棘颗粒的最佳含量测定方法。方法:采用正交设计L9(34)方法,对供试品溶液的提取确定水解温度、水解时间、萃取次数三因素,各因素确定三个水平,优选出最佳的提取方法。结果:将提取条件最终定为水解温度75℃,水解时间45分钟,萃取3次,较适合于检测操作。结论:采用该法后,由于样品取样量降低,用三氯甲烷萃取时,大部分水溶性杂质留在水解液中,供试品溶液具有澄清透明容易过滤等优点,可用于该品种的含量检测。
Objective: To screen out the best content determination method for Wuwei Seabuckthorn granules. Methods: The orthogonal design of L9(34) method was used to determine the hydrolysis temperature, hydrolysis time and extraction time for the extraction of the test solution. Three levels were determined for each factor, and the best extraction method was selected. Results: The extraction conditions were finally determined as the hydrolysis temperature of 75°C, the hydrolysis time of 45 minutes, and the extraction time of 3 times, which was suitable for the detection operation. Conclusion: After adopting this method, due to the decrease of the sampling quantity of the sample, most of the water-soluble impurities remain in the hydrolyzate when extracted with chloroform, and the sample solution has the advantages of being clear and transparent and easy to filter, and can be used for the content detection of this species. .