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采用ＰＣＲ技术、小型聚丙烯酰胺凝胶垂直电泳银染检测法，对１６９例云南汉族进行了Ｄ１Ｓ８０位点的ＶＮＴＲ扩增片段长度多态性（Ａｍｐ－ＦＬＰ）分析，发现了１８个等位基因，５１种基因型，其分布符合Ｈａｒｄｙ－Ｗｅｎｂｅｒｇ定律．片断大小分布于４００～７５０ｂｐ之间，基因频率为０．００３０～０．２６３３．杂合度为８７％，个人识别能力为０．９５８６，对９个家系的相关个体分析符合孟德尔定律．
A total of 169 alleles of Han population were detected by polymerase chain reaction (PCR) and silver polyacrylamide gel electrophoresis with silver staining. The VNTR amplified fragment length polymorphism (Amp-FLP) analysis of D1S80 locus showed that 18 alleles , 51 genotypes, the distribution of which is in accordance with Hardy-Wenberg’s law. Fragment size distribution in 400 ~ 750bp between the gene frequency of 0.0030 ~ 0.2633. The heterozygosity was 87% and the personal identification ability was 0.9586. The related individuals in 9 pedigrees were in accordance with Mendel’s law.