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目的:观察兔骨髓基质细胞(bonemarrowstromalcells,MSCs)的部分生物学特性及植入心肌后的存活状况。方法:密度梯度离心与贴壁粘附得到高纯度的兔下肢骨MSCs,建立体外培养体系,光镜、电镜、细胞周期等观察其生物学特性,4’,6-二脒基-2-苯吲哚(4’,6-diamidino-2-phenylindone,DAPI)标记培养的MSCs,植入心肌后检测植入细胞在6周内不同时相点的存活状况。结果:体外培养的MSCs显示良好的贴壁扩增生长能力,88%的培养细胞为静止期细胞,96.4%细胞为活细胞,电镜证明培养细胞显示幼稚细胞的结构。标记的MSCs植入心肌后至少生存6周并且植入细胞扩散融合于植入区宿主心肌中。结论:体外培养的MSCs显示幼稚细胞形态,标记的培养MSCs植入心肌后可以长期存活并扩散融合于宿主心肌。
Objective: To observe the biological characteristics of rabbit bone marrow stromal cells (MSCs) and their survival after implantation. METHODS: Rabbit bone marrow MSCs with high purity were isolated by density gradient centrifugation and adherent adhesion. The biological characteristics of rabbit bone were observed by light microscope, electron microscope and cell cycle. The cultured MSCs were labeled with indole (4 ’, 6-diamidino-2-phenylindone, DAPI), and the survival of the implanted cells at different time points after 6 weeks was detected. Results: MSCs cultured in vitro showed good adherent growth-promoting ability. 88% of the cultured cells were quiescent cells and 96.4% of the cells were viable cells. Electron microscopy showed that the cultured cells showed the structure of naive cells. The labeled MSCs survived for at least 6 weeks after implantation into the myocardium and the implanted cells diffused into the host myocardium in the implantation area. CONCLUSION: MSCs cultured in vitro show immature cell morphology. After labeled MSCs are implanted into the myocardium, they can survive long-term and diffuse into the host myocardium.