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目的 探讨大鼠局灶性脑缺血再灌注后不同时间、不同脑部位神经干细胞(NSC)原位激活的变化规律。方法 建立一侧大脑中动脉闭塞缺血再灌注模型,用5-溴脱氧尿苷(BrdU)标记脑内增殖细胞,免疫组化(SP法)技术检测大鼠缺血再灌注不同时间段缺血周边皮质、双侧侧脑室下区(SVZ)、海马齿状回下区(SGZ)具有增殖能力细胞BrdU表达变化。结果 对照组脑组织双侧SVZ、SGZ可见少量BrdU阳性标记细胞;单纯脑缺血2 h组脑组织BrdU阳性细胞数未见明显增加;再灌注3天组,缺血侧皮质、双侧SVZ和SGZBrdU阳性细胞明显增多(P<0.05),7天时达到高峰(P<0.001),11天时下降,且缺血侧与对侧相比,双侧SVZ呈对称分布,而SGZ以缺血侧增加为主(P<0.05)。结论 一侧大脑中动脉闭塞缺血再灌注可致成年大鼠脑内NSC原位激活,且NSC增殖于缺血后1周达到高峰,激活部位以双侧SVZ、缺血侧海马和周边皮质为主。
Objective To investigate the changes of in situ activation of neural stem cells (NSCs) in different brain regions at different time points after focal cerebral ischemia-reperfusion in rats. Methods A rat model of occlusion of middle cerebral artery occlusion (MCAO) was established. BrdU labeled brain cells were injected into the brain and immunohistochemistry (SP method) was used to detect the ischemia and reperfusion Peripheral cortex, bilateral subventricular zone (SVZ), hippocampal dentate gyrus (SGZ) proliferate cells BrdU expression changes. Results BrdU-positive cells were found in SVZ and SGZ of both groups in control group. No significant increase of BrdU positive cells was found in brain tissue at 2 h after cerebral ischemia in reperfusion group. In ischemic cortex and bilateral SVZ SGZBrdU positive cells were significantly increased (P <0.05), peaked at 7 days (P <0.001), decreased on the 11th day, and the bilateral SVZ was symmetrical in the ischemic and contralateral sides, while the SGZ increased in the ischemic side (P <0.05). Conclusion One side of middle cerebral artery occlusion ischemia reperfusion can cause NSC in the brain of adult rats in situ activation, and the NSC proliferation peaked at 1 week after ischemia, activation site to bilateral SVZ, ischemic hippocampus and peripheral cortex the Lord.