目的了解柳州市HIV亚型分布和原发性耐药情况。方法招募未经抗病毒治疗的柳州HIV感染者66名,采集外周静脉血,从血浆中提取HIV RNA,扩增pol基因并测序,将序列进行系统进化树分析和耐药性分析。结果获得60株序列,47株为CRF01_AE,占78.3%,12株为CRF07_BC,占20.0%,1株不能判断亚型。9株发生耐药性相关突变,其中2株可产生耐药性,原发性耐药的发生率为3.3%。结论当前柳州主要的HIV流行亚型是CRF01_AE和CRF07_BC,HIV原发性耐药的发生率处于国内一般水平。 Objective To understand the distribution of HIV subtypes and the primary drug resistance in Liuzhou. Methods Sixty-six HIV-infected patients without Liuzhou were enrolled. Peripheral venous blood was collected and HIV RNA was extracted from plasma. The pol gene was amplified and sequenced. The phylogenetic tree analysis and drug resistance analysis were performed. Results Sixty strains were obtained, of which 47 were CRF01_AE, accounting for 78.3%, 12 were CRF07_BC, accounting for 20.0%. One strain could not determine the subtype. Nine strains were resistant to mutations, of which two strains developed resistance and the incidence of primary resistance was 3.3%. Conclusions At present, the major HIV subtypes in Liuzhou are CRF01_AE and CRF07_BC. The prevalence of primary drug resistance in HIV is at the domestic level.