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【目的】研究不同浓度黄芪注射液对米非司酮诱导滋养细胞凋亡的影响。【方法】采用体外培养滋养细胞的方法,用60μmol/L米非司酮培养滋养细胞24、48 h后撤药,加入体积分数1%、0.1%、0.01%、0.001%黄芪注射液(分别含黄芪原药材20、2、0.2、0.02 mg/L),并检测各组细胞增殖、凋亡及凋亡蛋白Caspase-3表达情况。【结果】黄芪注射液对滋养细胞增殖率无显著改善(P>0.05),但可以显著降低细胞早期凋亡率(P<0.05或P<0.01),不同程度抑制Caspase-3蛋白表达。【结论】黄芪注射液对米非司酮诱导的滋养细胞凋亡具有一定的抑制作用。
【Objective】 To investigate the effects of Astragalus membranaceus injection on apoptosis of trophoblast cells induced by mifepristone. 【Method】 Trophoblastic cells were cultured in vitro and cultured for 24 and 48 h with 60 μmol / L mifepristone. The cells were treated with 1%, 0.1%, 0.01% and 0.001% astragalus injection respectively Astragalus herbs 20,2,0.2,0.02 mg / L), and detected the proliferation, apoptosis and expression of Caspase-3 in each group. 【Results】 Astragalus injection had no significant effect on the proliferation rate of trophoblast cells (P> 0.05), but could significantly reduce the early apoptosis rate (P <0.05 or P <0.01) and inhibit the expression of Caspase-3 protein to some extent. 【Conclusion】 Astragalus injection can inhibit the apoptosis of trophoblast cells induced by mifepristone.