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目的：研究生理脉动切应力对血管内皮细胞一氧化氮产生的影响。材料与方法：利用本室已建立的平行板模拟血流循环装置，在模拟生理脉动切应力（１９． ２９，５６． ２５ ｄｙｎｅｓ／ｃｍ２）分别作用于培养人脐静脉内皮细胞 ０，１，２，４，６，１０，２４ ｈ后，用一氧化氮微量测定法测定循环液中一氧化氮的含量。结果：在两组模拟生理脉动切应力的作用下，于２ｈ时一氧化氮释放出现一峰值，之后逐渐下降，并且内皮细胞释放一氧化氮的量随切应力的增大而增加。结论：生理脉动切应力对血管内皮细胞一氧化氮的产生具有调节作用，可能对血管动脉粥样硬化的形成与发展中，临床手术前后发生的血管痉挛与血栓形成等增多有重要影响。
Objective: To study the effect of physiological pulse shear stress on nitric oxide production in vascular endothelial cells. MATERIALS AND METHODS: Using the parallel plate simulated blood circulation device established in our laboratory, we induced human umbilical vein endothelial cells 0, 1, 2 at simulated physiological pulsation shear stress (19. 29, 56. 25 dynes / cm2) , 4,6,10,24 h, nitric oxide microassay determination of circulating fluid nitric oxide content. Results: Under the action of simulated physiological pulsating shear stress, the release of nitric oxide showed a peak at 2 hours and then decreased gradually. The amount of nitric oxide released by endothelial cells increased with the increase of shear stress. CONCLUSION: Physiological pulsating shear stress has a regulatory effect on the production of nitric oxide in vascular endothelial cells, which may have an important effect on the formation and development of atherosclerosis and the increase of vasospasm and thrombosis before and after clinical surgery.