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[目的]从光谱学角度分析神经干细胞(NSCs)与神经细胞(NCs)的光谱区别,为临床应用提供一定的试验参考依据。[方法]通过体外培养NSCs和NCs,应用激光共焦显微拉曼光谱仪测定2种细胞的拉曼光谱。[结果]NSCs与NCs的拉曼特征峰主要出现在600～1800 cm-1的波数范围和2850～3 000 cm-1波数范围内,拉曼光谱的峰位和相对峰强存在一定差异。NSCs的拉曼峰646、2917 cm-1与NCs的拉曼峰652、2908 cm-1处存在较大差异。NCs核中B型DNA胸腺嘧啶和酪氨酸含量相对更丰富。NSCs核中具有NO2成分,而NCs核中NO2较少或没有。NSCs与NCs核内部酰胺Ⅲ、C-H键、类胡萝卜素、C-H键、CH2键、CH3基团的结构不发生明显变化。NSCs较NCs的DNA、酪氨酸、蛋氨酸、腺嘌呤、苯基丙氨酸、胡萝卜素、酰胺Ⅲ等含量更丰富,其中以胡萝卜素和酰胺Ⅲ的相对含量最高。[结论]从光谱学角度研究NSCs与NCs,是一种新的干细胞研究方法。
[Objective] To analyze the spectral difference between neural stem cells (NSCs) and nerve cells (NCs) from the perspective of spectroscopy and provide some experimental reference for clinical application. [Methods] Raman spectra of two kinds of cells were determined by laser confocal microscopy Raman spectroscopy in vitro culture of NSCs and NCs. [Result] The Raman peaks of NSCs and NCs mainly appeared in the wave number range of 600-1800 cm-1 and in the wave number range of 2850-3000 cm-1. There were some differences in the Raman peak position and relative peak intensity. There was a big difference between the Raman peaks of 646 and 2917 cm-1 of NSCs and the Raman peaks of 652 and 2908 cm-1 of NCs. B-type DNA in the nucleus of NCs is relatively abundant in thymine and tyrosine. There are NO2 components in the nucleus of NSCs, but NO2 in nuclei of NCs is less or no. The structures of amide Ⅲ, C-H bond, carotenoid, C-H bond, CH2 bond and CH3 group in NSCs and NCs nuclei did not change obviously. NSCs were more abundant than NCs in terms of DNA, tyrosine, methionine, adenine, phenylalanine, carotene and amide III, with the highest relative content of carotene and amide III. [Conclusion] The study of NSCs and NCs from the perspective of spectroscopy is a new method of stem cell research.