论文部分内容阅读
目的应用siRNA沉默SMMC7721细胞MAPK p42,并观察其对增殖和凋亡的影响。方法应用SilencerTMsiRNA构建试剂盒合成2条MAPK p42siRNAs和一条随机对照siRNAs,用脂质体LipofectaminTM 2000将其转染入肝癌SMMC-7721细胞株。应用Western blot检测MAPK p42表达,MTT法检测细胞增殖情况,流式细胞仪分析细胞周期及其细胞凋亡。结果与对照相比,MAPK p42siRNA明显抑制MAPK p42表达。MAPK p42siRNA抑制了SMMC-7721细胞的生长,将细胞阻滞于细胞周期S期,进而诱导了细胞凋亡的发生。结论 MAPK p42靶向siRNA可能为肝癌基因治疗的重要手段。
Objective To silence MAPK p42 in SMMC7721 cells with siRNA and observe its effect on proliferation and apoptosis. Methods Two MAPK p42siRNAs and one control siRNAs were synthesized by SilencerTM siRNA kit and transfected into SMMC-7721 hepatoma cell line with LipofectaminTM 2000. The expression of MAPK p42 was detected by Western blot. The cell proliferation was detected by MTT assay. Cell cycle and apoptosis were analyzed by flow cytometry. Results Compared with the control, MAPK p42siRNA significantly inhibited MAPK p42 expression. MAPK p42siRNA inhibits the growth of SMMC-7721 cells, arrests the cells in the S phase of the cell cycle, and then induces apoptosis. Conclusion MAPK p42 targeting siRNA may be an important means of gene therapy for hepatocellular carcinoma.