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目的:比较HSPgp96与LPS对巨噬细胞免疫功能的激活作用。方法:培养小鼠腹腔巨噬细胞,分组用PBS、HSPgp96和LPS诱导后,在不同时间用酶法测定NO生成量。按效靶比10:1加入H22肝癌细胞,24h后靶细胞进行单细胞凝胶电泳,染色观察。在不同时间用激光扫描共聚焦显微镜观测巨噬细胞MHC-Ⅱ和CD86的表达。结果:LPS和HSPgp96促进巨噬细胞NO生成的作用相当,并随作用时间延长而增加。巨噬细胞攻击后的靶细胞呈现DNA损伤后的彗星现象。LPS和HSPgp96均促进巨噬细胞MHC-Ⅱ和CD86的表达,但HSPgp96诱导组表达更快更强。结论:与LPS相比,HSPgp96活化小鼠腹腔巨噬细胞细胞毒活性的作用相当,而诱导抗原提呈功能的作用更强。
Objective: To compare the activation of HSPgp96 and LPS on macrophage immune function. Methods: Mouse peritoneal macrophages were cultured, and after grouping with PBS, HSPgp96 and LPS, NO production was measured enzymatically at different times. H22 hepatocarcinoma cells were added according to the effective target ratio of 10: 1, and the target cells were examined by single cell gel electrophoresis after 24 hours. Laser scanning confocal microscopy was used to observe the expression of MHC-Ⅱ and CD86 in macrophages at different times. Results: The effect of LPS and HSPgp96 on NO production by macrophages was comparable and increased with time. Target cells after macrophage attack show cometary phenomenon after DNA damage. Both LPS and HSPgp96 promoted the expression of MHC-II and CD86 in macrophages, but the expression in HSPgp96-induced group was faster and stronger. CONCLUSION: Compared with LPS, HSPgp96 activates the peritoneal macrophages in mice with comparable cytotoxicity and induces the antigen presenting function.