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目的:探讨hTERC基因与宫颈病变的关系,协助临床筛查宫颈癌前病变及宫颈癌。方法:取20例正常妇女及82例宫颈病变患者的宫颈脱落细胞,其中CINⅠ11例,CINⅡ26例、CINⅢ22例、宫颈癌23例,用荧光原位杂交方法(FISH)检测hTERC基因的扩增情况。结果:CINⅠ、CINⅡ、CINⅢ及宫颈癌患者的hTERC扩增阳性率分别为9.1%、57.7%、100%、95.7%,CINⅢ及宫颈癌的hTERC基因的扩增率明显高于其它组,hTERC预测宫颈高度病变(CINⅡ/Ⅲ及宫颈癌)的敏感度、特异度分别为83.10%、96.78%。结论:随着组织学病变程度的增加,hTERC的扩增数逐渐增高,FISH技术可协助临床提高宫颈病变的筛查率。
Objective: To investigate the relationship between hTERC gene and cervical lesions and to help clinical screening of cervical precancerous lesions and cervical cancer. METHODS: Twenty exfoliated cervical cells were obtained from 20 normal women and 82 cervical lesions. Among them, 11 cases of CINⅠ, 26 cases of CINⅡ, 22 cases of CINⅢ and 23 cases of cervical cancer were detected by fluorescence in situ hybridization (FISH) to detect the amplification of hTERC gene. Results: The positive rates of hTERC amplification in patients with CINⅠ, CINⅡ, CINⅢ and cervical cancer were 9.1%, 57.7%, 100% and 95.7% respectively. The hTERC gene amplification rate in CINⅢ and cervical cancer was significantly higher than those in other groups. The sensitivity and specificity of cervical hyperplasia (CINⅡ / Ⅲand cervical cancer) were 83.10% and 96.78% respectively. Conclusion: With the increase of histological lesion, the number of hTERC amplification gradually increased, FISH can help clinically improve the screening rate of cervical lesions.