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采用四氯化碳(CCl _4)中毒性大鼠肝损害模型,用薄层层析和定磷法检测四组大鼠肝脏膜磷脂成份磷脂酰胆碱(Phosphatidylcholne PC),鞘磷脂(Sphingomyelin,SM)在总磷脂中的相对百分含量,并测定肝脏组织内的γ-谷氨酰转肽酶(γGT)的活性。结果表明:在CCl_4染毒后12和24小时,PC和SM明显降低,48小时又有所上升。但PC尽管回升,与对照组比较仍低(P<0.05),而SM和对照组之间无显著性差别。γ-GT的活性在12、24、48h分别为对照组的29％、44.7％、147％,与对照组之间的差别有显著性。这提示CCl_4染毒后,PC和SM的变化有类似于癌变过程中PC扣SM变化的趋势,可能具有相似的调控作用。
The liver damage model of rats with carbon tetrachloride (CCl_4) poisoning was established. Phosphatidylcholne PC, Sphingomyelin (SM) ) In total phospholipids, and the activity of γ-glutamyl transpeptidase (γGT) in liver tissue was measured. The results showed that at 12 and 24 hours after CCl_4 treatment, PC and SM decreased significantly, and then increased after 48 hours. However, the recovery of PC was still low (P <0.05) compared with the control group, but there was no significant difference between SM and the control group. The activity of γ-GT was 29%, 44.7% and 147% respectively at 12, 24 and 48h, which was significantly different from the control group. This suggests that the changes of PC and SM after CCl_4 exposure have a tendency similar to that of PC with SM during carcinogenesis and may have similar regulation.