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用小鼠骨髓瘤细胞系SP 2/0与新鲜癌组织匀浆免疫的Balb/C小鼠脾细胞融合制备抗人胰腺癌单克隆抗体。经间接荧光抗体法和免疫组织化学法筛选及有限稀释法克隆化后,获得一株产生与正常人体组织不反应,与人胰腺癌反应率高的抗人胰腺癌单克隆抗体的杂交瘤细胞株。
Anti-human pancreatic cancer monoclonal antibodies were prepared by fusing Balb / C mouse spleen cells immunized with fresh myeloma tissue spleen cells with mouse myeloma cell line SP2 / 0. After cloning by indirect fluorescent antibody method, immunohistochemical method and limiting dilution method, a hybridoma cell line producing monoclonal antibody against human pancreatic cancer with high reactivity with human pancreatic cancer without any reaction to normal human tissues was obtained .