目的采用HPLC法测定L-(+)-扁桃酸的光学纯度。方法采用Chirlcel OJ-H手性色谱柱,以正己烷-乙醇-三氟乙酸(96∶4∶0.3)为流动相,检测波长220 nm,流速1.0 m L·min-1,柱温30℃。结果 L-(+)-扁桃酸与D-(-)-扁桃酸的分离良好,L-(+)-扁桃酸0.2~1.2×103μg·m L-1与峰面积的线性关系良好(r=0.9998),检测限为0.4 ng(S/N=3),定量限为1.6 ng(S/N=10)。结论所用方法快速、准确、灵敏,适于L-(+)-扁桃酸光学纯度的测定。 Objective To determine the optical purity of L - (+) - mandelic acid by HPLC. Methods The Chiralcel OJ-H chiral column was used with a mobile phase of n-hexane-ethanol-trifluoroacetic acid (96: 4: 0.3) at a wavelength of 220 nm and a flow rate of 1.0 m L · min- Results The separation of L - (+) - mandelic acid and D - (-) - mandelic acid was good. The linear relationship of L - (+) - mandelic acid 0.2 ~ 1.2 × 103μg · m L -1 with the peak area was good (r = 0.9998). The limit of detection was 0.4 ng (S / N = 3). The limit of quantification was 1.6 ng (S / N = 10). Conclusion The method is rapid, accurate and sensitive and suitable for the determination of optical purity of L - (+) - mandelic acid.