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Objective: To investigate the cytotoxic effects and the potential rnmechanisms of crebanine N-oxide in SGC-7901 gastric rnadenocarcinoma cells.rnMethods: The cytotoxicity of crebanine N-oxide was evaluated by rn3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide rnassay and cellular morphology was observed under a microscope.rnCell apoptosis was determined by flow cytometry using propidium rniodide staining. The expression levels of apoptotic-related proteins,rncleaved caspase-3, cytochrome C, p53 and Bax, and autophagyrelated rnproteins p62, beclin1 and LC3 were detected by West rnblotting assays.rnResults: Crebanine N-oxide treatment significantly inhibited the rnproliferation of SGC-7901 cells in a dose-dependent and timedependent rnmanner via induction of G2-phase cell cycle arrest,rnapoptosis, and autophagy in SGC-7901 cells.rnConclusions: Crebanine N-oxide could inhibit the growth of gastric rncancer cells by promoting apoptosis and autophagy and could be rnused as a potential agent for treating gastric cancer.