目的 :观察苯海索对谷氨酸引起的神经细胞内游离钙 ([Ca2 + ]i)含量的影响。方法 :体外培养大鼠皮层神经细胞 ,加入谷氨酸观察细胞内 [Ca2 + ]i,培养液一氧化氮 (NO)含量的变化及苯海索的干预作用。结果 :加入谷氨酸后细胞内 [Ca2 + ]i、细胞死亡数、NO含量显著升高 ,苯海索各浓度显著抑制 [Ca2 + ]i及 NO的升高 ,减少细胞死亡。结论 :苯海索对谷氨酸引起的神经细胞内 [Ca2 + ]i、NO含量的升高具有抑制作用 ,其机制可能与钙拮抗作用有关 Objective: To observe the effect of trihexyphenidyl on glutamate-induced intracellular free calcium ([Ca2 +] i) in nerve cells. Methods: Cultured rat cortical neurons were cultured in vitro. Glutamate was used to observe the intracellular [Ca2 +] i and the content of nitric oxide (NO) in culture fluid and the effect of trihexyphenidyl. RESULTS: After addition of glutamic acid, intracellular [Ca2 +] i, cell death number and NO content were significantly increased. The concentrations of triptorelin significantly inhibited the increase of [Ca2 +] i and NO, and decreased cell death. CONCLUSION: Phenoxybenzin inhibits the increase of [Ca2 +] i and NO in glutamate-induced neurons, and its mechanism may be related to calcium antagonism