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目的 :用乙型肝炎病毒 (HBV ,HepatitisBVirus)表面抗原DNA疫苗对小鼠进行基因免疫 ,观察主要脏器病理形态改变 ,探讨该DNA疫苗是否对肝、肾、脾等有损伤作用。方法 :用乙型肝炎表面抗原 (HBsAg)DNA疫苗经肌肉注射法免疫小鼠 ;ELISA法检测小鼠血清抗HBsAg抗体水平。免疫 12周后取小鼠肝、肾、脾等组织 ,观察其病理形态改变情况。 结果 :DNA疫苗接种l周后 ,免疫组小鼠血清中可检测到抗HBsAg抗体 ,12周后多只小鼠脾脏明显增大 ,肝、肾组织切片染色观察 ,可见免疫组小鼠的肝、肾组织均有不同程度的病理改变。结论 :HBsAgDNA疫苗可诱导小鼠机体产生特异性抗体 (可诱发全面、持久的免疫应答 )持续 12周以上 ,同时伴有脾脏肿大和肝肾损伤提示 ,该DNA疫苗表达产物与免疫小鼠脏器病变有一定的关系 ,对其临床应用前景值得进一步探索
OBJECTIVE: To immunize mice with DNA vaccine against hepatitis B virus (HBV) surface antigen and observe the pathological changes of major organs to investigate whether the DNA vaccine can damage the liver, kidney and spleen. Methods: Mice were immunized by intramuscular injection of Hepatitis B surface antigen (HBsAg) DNA vaccine and serum anti-HBsAg antibody levels by ELISA. After 12 weeks of immunization, the liver, kidney, spleen and other tissues were taken and the pathological changes were observed. Results: After 1 week of DNA vaccination, anti-HBsAg antibody could be detected in the sera of immunized mice. After 12 weeks, the spleens of multiple mice were significantly increased. The liver and kidney tissue sections were stained. The liver, Renal tissue has varying degrees of pathological changes. Conclusion: HBsAg DNA vaccine can induce specific antibody (induced a comprehensive and long-lasting immune response) in mice for more than 12 weeks, accompanied by splenomegaly and liver and kidney damage, suggesting that the DNA vaccine product and immune mouse organ Lesions have a certain relationship, its clinical application is worth further exploration