本文采用薄层层析法进行分析，结果表明：正红菇含有多种甾醇，其中麦角固醇已被确定，对麦角固醇进行提取分析，其含量为０９５％（ｍｇ／１００ｍｇ）。采用不同提取法，其结果不加酶提取麦角固醇的含量高于加酶，不加酶其含量为０９５％（ｍｇ／１００ｍｇ），加酶为０６７％（ｍｇ／１００ｍｇ）。多糖提取是酶法优于常规热水浸提法。热水浸提多糖为２７４％（ｇ／１００ｇ），双酶浸提为２８５％（ｇ／１００ｇ），三酶浸提法为３１９％（ｇ／１００ｇ）。 In this paper, the TLC analysis was conducted. The results showed that: Zhengrui contains a variety of sterols, including ergosterol has been identified, ergosterol extraction analysis, the content of 0  95% (mg / 100mg). Using different extraction methods, the results showed that the content of ergosterol without enzyme was higher than that with enzyme. The content of ergosterol without enzyme was 095% (mg / 100mg) and the enzyme was 0.67% (mg / 100mg). Polysaccharide extraction is better than conventional hot water extraction method. Hot water extraction of polysaccharides was 274% (g / 100g), double enzyme extraction was 285% (g / 100g), three enzyme extraction method was 319% (g / 100g).