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乙型肝炎病毒(HBV)的主要表面抗原(HBsAg)是由226个氨基酸(P24)的多肽及其糖苷化形式(GP27)所组成。最近又测出了微量的两对蛋白,它们是氨基酸末端延伸55个氨基酸构成的Pre-S_2蛋白(GP33和GP36)和延伸多达108个氨基酸构成的Pre-S_1蛋白(P39和GP42)。Pre-S_2蛋白质只在HBV感染的急性期产生,其作用可能是介导病毒吸附于细胞上。但HBV感染后Pre-S_1表达的时间过程一直未测出。为了确定诊断HBV感染或者HBV感染的预后或者两者Pre-S_1-HBsAg及相应抗体的重要性,作者用带有pPlc24质粒克隆的大肠杆菌合成的MS2聚合酶-Pre-S_1融合蛋白,再经理化法提取Pre-S_1融合蛋白并免疫家兔,获得针对Pre-S_1特异的氨基酸序列
The major surface antigen (HBsAg) of Hepatitis B virus (HBV) consists of a 226 amino acid (P24) polypeptide and its glycosylated form (GP27). Recently, two pairs of proteins were detected in small amounts. They are Pre-S 2 (GP33 and GP36) consisting of 55 amino acids extending from the amino acid end and Pre-S 1 proteins (P39 and GP42) extending up to 108 amino acids. Pre-S 2 protein is only produced during the acute phase of HBV infection, and its role may be to mediate the virus’s adsorption on the cells. However, the time course of Pre-S 1 expression after HBV infection has not been detected. In order to determine the importance of predicting the prognosis of HBV infection or HBV infection, or both Pre-S_1-HBsAg and corresponding antibodies, the authors used an E. coli-synthesized MS2 polymerase-Pre-S_1 fusion protein with the pPlc24 plasmid clones Method to extract Pre-S_1 fusion protein and immunized rabbits to obtain Pre-S_1-specific amino acid sequence