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将枣树不同品种的不同器官及其部位的外植体接种在含有不同激素的培养基中培养.对其愈伤组织诱导条件进行了研究.结果表明;灌阳长枣(中国)和普通枣(日本)为较适宜组培的品种.胚、嫩枝顶部、芽为适宜组培的器官;以MS(NO3)作基本培养基、并添加3μmol左右Zeatin和约30μmolNAA或IAA可以获得数量较多、质量较好的愈伤组织;叶所产生的愈伤组织颜色较深,数量也较少,并在初代培养时易产生不定根.
The different organs of jujube and its parts of different explants inoculated with different hormones in culture medium. The callus induction conditions were studied. The results showed that Guanyang long date (China) and common date (Japan) were more suitable tissue culture varieties. Embryo, top of shoots and shoots were suitable tissues for tissue culture. MS (NO3) was used as basic medium, and about 3μmol of Zeatin and about 30μmol of NAA or IAA could be used to obtain more callus with better quality. The callus darker in color and less in number, and easy to produce adventitious roots in the initial culture.