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GM-CSF工程菌W3100经发酵培养后,将菌体低渗裂解,经流水柱─分子筛─阴离子交换树脂的一系列层析,最后得到纯化的GM-CSF,其纯度为98%以上,比活性大于20×107。
GM-CSF engineered bacteria W3100 after fermentation culture, the cell hypotonic lysis, a series of column chromatography - anion exchange resin chromatography, and finally purified GM-CSF, the purity of more than 98%, specific activity Greater than 20 × 107.