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H9020175是一个通过杂交和回交选育的普通小麦华山新麦草易位系,接种鉴定表明其对条锈病具有优良抗性。遗传学分析证明易位系H9020175的抗条锈性是由单基因控制的显性性状,抗性基因来自于华山新麦草,暂定名为YrHua。为了标记这个来自华山新麦草的抗条锈病基因,利用H9020175与感病小麦品种铭贤169杂交,建立了F2分离群体。应用81对AFLP引物对119个经条锈菌生理小种CY30接种鉴定的F2单株进行了分析,结果得到两个与YrHua基因连锁的AFLP标记PM14(301)和PM42(249),遗传距离分别为5.4cM和2.7cM,并分别位于目标基因的两侧。将标记片段克隆、测序后,根据序列信息和酶切位点多态性设计特异性引物,将AFLP标记PM14(301)转换成了简单的PCR标记。研究结果为标记辅助育种提供了分子选择工具,同时也为进一步精细定位和图位克隆YrHua基因奠定了基础。
H9020175 is a New Wheat (T. huashanica) translocation line bred by crossing and backcross. Inoculation identification showed that it has excellent resistance to stripe rust. Genetic analysis proves that the stripe rust resistance of the translocation line H9020175 is a dominant gene controlled by a single gene. The resistance gene is derived from P. huashanica and tentatively named as YrHua. In order to mark this stripe rust resistance gene from P. huashanica, F2 segregation population was established by crossing H9020175 with susceptible wheat cultivar Mingxian169. Application of AFLP primer pair analysis of 119 F1 F2 progeny identified by the CY30 inoculation showed that two AFLP markers PM14 (301) and PM42 (249) linked to the YrHua gene were obtained. The genetic distances were 5.4cM and 2.7cM, respectively, located on each side of the target gene. The marker fragment was cloned and sequenced. According to the sequence information and the restriction site polymorphism, AFLP marker PM14 (301) was transformed into a simple PCR marker. The results provided a molecular selection tool for marker-assisted breeding and laid the foundation for further fine mapping and mapping of YrHua gene.