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31只健康豚鼠分为哮喘组、地塞米松干预组及对照组。测定各组豚鼠肺泡灌洗液细胞成分 ,应用RT PCR方法检测支气管组织白细胞介素 5(IL 5)和白细胞介素 1 0 (IL 1 0 )mRNA表达强度。结果显示 ,哮喘组豚鼠肺泡灌洗液中嗜酸性粒细胞 (EOS)百分比、IL 5mRNA表达量均显著高于地塞米松治疗组及对照组 ,而IL 1 0mRNA表达则相反。IL 5mRNA表达与EOS百分比呈正相关 ,而与IL 1 0mRNA表达呈负相关。说明EOS ,IL 5以正相关参与了哮喘发病。哮喘时IL 1 0表达受抑制 ,这可能是炎症细胞因子IL 5合成增多的主要原因之一。糖皮质激素治疗哮喘的作用机制之一 ,可能与其提高IL 1 0及下调IL 5表达水平有关
31 healthy guinea pigs were divided into asthma group, dexamethasone intervention group and control group. The cellular components in the bronchoalveolar lavage fluid of guinea pigs in each group were determined. The expression of interleukin 5 (IL 5) and interleukin 10 (IL 1 0) mRNA in bronchial tissues was detected by RT-PCR. The results showed that the percentage of eosinophils (EOS) and the expression of IL-5 mRNA in bronchoalveolar lavage fluid of asthmatic group were significantly higher than that of dexamethasone treatment group and control group, while the expression of IL-10 mRNA was opposite. IL 5 mRNA expression was positively correlated with the percentage of EOS, but negatively correlated with IL 1 0 mRNA expression. This shows that EOS and IL-5 are involved in the pathogenesis of asthma with positive correlation. The suppression of IL-10 expression in asthma may be one of the main reasons for the increased synthesis of inflammatory cytokines IL-5. One of the mechanisms of action of glucocorticoids on asthma may be related to the increase of IL 1 0 and the downregulation of IL 5 expression