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背景:近年来研究发现托吡酯能阻断电压依赖性钠通道;增强γ氨基丁酸(Gamma-aminobutyricacid,GABA)能的活性;阻滞α-氨基-3-羟基-5-甲基-4-异恶唑丙酸/海人藻酸(α-amino-3-hydroxy-5-methy-lisoxazole-4-propionicacid/kainicacid,AMPA/KA)型谷氨酸受体的作用,从而推测其有神经保护作用。目的:观察托吡酯对大鼠局灶脑缺血的神经保护作用。设计:完全随机设计、对照实验研究。地点与材料:地点为上海第二医科大学附属仁济医院神经生物实验室。健康雄性大鼠50只Sprague-Dawley,体质量280～350g,随机分为3组,购自中科院上海实验动物中心。干预:以腔内线栓法制作大鼠局灶脑缺血模型,将50只Sprague-Dawley大鼠单纯随机分为对照组(10只)、40mg/kg治疗组(20只)、80mg/kg治疗组(20只),在缺血30min后一次腹腔注射托吡酯,对照组注射生理盐水。主要观察指标:分别在4,24h后观察神经功能评分,24h后干/湿重法测定脑组织含湿量、氯化三苯基四氮唑(2,3,5-triphenyltetrazoliumchlo-ride,TTC)染色观察测定梗死体积百分比。结果:①24h后神经功能评分显示,无论是40mg/kg组(3.20±0.52)还是80mg/kg组(2.70±0.47),分别与4h(40mg/kg,3.90±0.31;80mg/kg,3.80±0.41)相比有非常显著(P<0.01)的差异,两治疗组24h的评分与对照组的?
BACKGROUND: In recent years, it has been found that topiramate can block the voltage-dependent sodium channel, enhance the activity of GABA, block the effects of α-amino-3-hydroxy-5-methyl- The effect of glutamate receptors on α-amino-3-hydroxy-5-methy-lisoxazole-4-propionic acid / kainic acid (AMPA / KA) . Objective: To observe the neuroprotective effect of topiramate on focal cerebral ischemia in rats. Design: Completely randomized design, controlled experimental study. Venue and Materials: The location is the Neurobiology Laboratory of Renji Hospital affiliated to Shanghai Second Medical University. Fifty healthy male Sprague-Dawley rats weighing 280-350 g were randomly divided into three groups and purchased from Shanghai Experimental Animal Center of Chinese Academy of Sciences. Intervention: A rat model of focal cerebral ischemia was established by intraluminal suture method. 50 Sprague-Dawley rats were randomly divided into control group (n = 10), 40 mg / kg treatment group (n = 20) and 80 mg / kg (N = 20). Topiramate was injected intraperitoneally 30 min after ischemia and normal saline was injected into the control group. MAIN OUTCOME MEASURES: Nerve function scores were observed after 4 and 24 hours, respectively. After 24 hours, the wet and dry weight of the brain tissue were measured. The contents of TTC (2,3,5-triphenyltetrazolium chloride) Staining Observations Percentage of infarct volume. Results: ①After 24 hours, the scores of neurological function showed no significant difference between the groups of 40mg / kg (3.20 ± 0.52) or 80mg / kg (2.70 ± 0.47) and 4h (40mg / kg, 3.90 ± 0.31; 80mg / kg, 3.80 ± 0.41 ) Compared with a significant (P <0.01) difference between the two treatment groups 24h score and the control group?